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Single-cell analysis using an Inductively Coupled Plasma-Mass Spectrometer (SC-ICP-MS) is enhancing the fundamental understanding in cellular biology, oncology and drug discovery. SC-ICP-MS provides for the quantification of metals in individual biological cells and is used as a research tool to allow scientists to study disease aetiology, better understand diseased cell states and develop new drug treatments to give better patient outcomes.
In single-cell analysis, the sample introduction system converts a continuous stream of biological cells efficiently into an aerosol of individual cells and transfers the aerosol into the Inductively Coupled Plasma of the ICP-MS for analysis. A critical aspect of the sample introduction system is the cells must remain intact and unruptured during the nebulization process to ensure each individual cell produces a single ion burst inside the plasma that can be transferred into the mass analyser of the ICP-MS for quantification.
Conventional ICP-MS spray chambers have low transport efficiencies (typically < 5% TE) that filter out larger droplets ( > 5µm) from the nebulizer aerosol. As most cell lines of interest are larger than 5µm, the secondary filtering mechanism of conventional spray chamber designs rejects a high proportion of the cells from passing to the plasma. To overcome this significant deficiency the Glass Expansion spray chamber design for single cell analysis is different from conventional ICP-MS spray chambers.
Part Number ICP-MS SC-SIS Kits KT-1155 Single-Cell Sample Introduction System for Agilent ® ICP-MS KT-1172 Single-Cell Sample Introduction System for Thermo ® ICP-MS KT-1172 Single-Cell Sample Introduction System for TOFWERK icpTOF KT-1184 Single-Cell Sample Introduction for PerkinElmer ® NexION 1000/2000/5000 KT-1204 Single-Cell Sample Introduction for PerkinElmer ® NexION 300/350 KT-1205 Single-Cell Sample Introduction System for NU ATTOM KT-1213 Single-Cell Sample Introduction for Thermo ® X Series KT-1215 Single-Cell Sample Introduction System for Thermo ® Neptune/Element
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have been using the Glass Expansion Single Cell Sample Introduction System in several studies including cells and Au NPs. Regarding the Au NPs, depending on operating conditions, transport efficiencies ranging between 60 and 95% were achieved.
ICP manufacturer - Europe
We tried the configuration you provided us, and the single cell sample introduction worked perfectly. We are tremendously grateful for your help.
University laboratory – Spain