NEWS
Figure 5. Stained PBMC acquired on a CyTOF2 mass cytometer using a conventional CyTOF
nebulizer and the prototype MircoMist nebulizer. The cell concentration, flow rate and total
acquisition time were kept constant. The resulting data were visualized using viSNE and some
major immune subsets were manually annotated on the map on the basis of canonical patterns of
surface marker expression.
Conventional CyTOF nebulizer
Prototype MicroMist nebulizer
Conclusions
The Glass Expansion prototype MicroMist nebulizer at optimized
operating conditions for Mass Cytometry produced excellent
data quality and single cell staining. Using a sample flow rate
of 45 μL/min, the prototype MicroMist nebulizer produced a
cell transmission efficiency of 33%. The prototype MicroMist
nebulizer provides comparable performance to the standard Mass
Cytometer nebulizer, but owing to the VitriCone capillary has the
advantages of outstanding analytical reproducibility and improved
resistance to blockages. The LC fittings kit provided a zero dead
volume connection for best sensitivity and low carry-over. Further
experiments are planned where the sample flow rate will be varied in
the range of 30 to 40 μL/min to improve cell transmission efficiency.
The TruFlo Sample Monitor serves as an essential component to
the CyTOF & Helios instruments so that the sample flow rate is
continuously and accurately monitored, ensuring the quality of cell
transmission efficiency.
www.geicp.com Glass Expansion Newsletter | Issue 41 4
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