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GE Newsletter October 2016

NEWS Figure 5. Stained PBMC acquired on a CyTOF2 mass cytometer using a conventional CyTOF nebulizer and the prototype MircoMist nebulizer. The cell concentration, flow rate and total acquisition time were kept constant. The resulting data were visualized using viSNE and some major immune subsets were manually annotated on the map on the basis of canonical patterns of surface marker expression. Conventional CyTOF nebulizer Prototype MicroMist nebulizer Conclusions The Glass Expansion prototype MicroMist nebulizer at optimized operating conditions for Mass Cytometry produced excellent data quality and single cell staining. Using a sample flow rate of 45 μL/min, the prototype MicroMist nebulizer produced a cell transmission efficiency of 33%. The prototype MicroMist nebulizer provides comparable performance to the standard Mass Cytometer nebulizer, but owing to the VitriCone capillary has the advantages of outstanding analytical reproducibility and improved resistance to blockages. The LC fittings kit provided a zero dead volume connection for best sensitivity and low carry-over. Further experiments are planned where the sample flow rate will be varied in the range of 30 to 40 μL/min to improve cell transmission efficiency. The TruFlo Sample Monitor serves as an essential component to the CyTOF & Helios instruments so that the sample flow rate is continuously and accurately monitored, ensuring the quality of cell transmission efficiency. www.geicp.com Glass Expansion Newsletter | Issue 41 4


GE Newsletter October 2016
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